Mastino may refer to:
- The rechristened name of the British boat Empire Becky
Dogs
- Mastino Napoletano, one of the names for the Neapolitan Mastiff breed of dog
Personas
- Bruiser Mastino, a wrestling persona performed by Mike Hallick and Glenn Jacobs
- Former member of the band Punkreas
People
- Attilio Mastino [it] (born 1949), rector for the University of Sassari
- F. Mastino, midfielder for FC Baulmes
- Mastino della Scala:
- Mastino I della Scala (died 1277)
- Mastino II della Scala (1308–1351)
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