CCA tRNA nucleotidyltransferase

CCA tRNA nucleotidyltransferase (EC 2.7.7.72, CCA-adding enzyme, tRNA adenylyltransferase, tRNA CCA-pyrophosphorylase, tRNA-nucleotidyltransferase, transfer-RNA nucleotidyltransferase, transfer ribonucleic acid nucleotidyl transferase, CTP(ATP):tRNA nucleotidyltransferase, transfer ribonucleate adenylyltransferase, transfer ribonucleate adenyltransferase, transfer RNA adenylyltransferase, transfer ribonucleate nucleotidyltransferase, ATP (CTP):tRNA nucleotidyltransferase, ribonucleic cytidylic cytidylic adenylic pyrophosphorylase, transfer ribonucleic adenylyl (cytidylyl) transferase, transfer ribonucleic-terminal trinucleotide nucleotidyltransferase, transfer ribonucleate cytidylyltransferase, ribonucleic cytidylyltransferase, -C-C-A pyrophosphorylase, ATP(CTP)-tRNA nucleotidyltransferase, tRNA adenylyl(cytidylyl)transferase, CTP:tRNA cytidylyltransferase) is an enzyme with systematic name CTP,CTP,ATP:tRNA cytidylyl,cytidylyl,adenylyltransferase. This enzyme catalyses the following chemical reaction

a tRNA precursor + 2 CTP + ATP

\rightleftharpoons

a tRNA with a 3' CCA end + 3 diphosphate (overall reaction)

(1a) a tRNA precursor + CTP

\rightleftharpoons

a tRNA with a 3' cytidine end + diphosphate

(1b) a tRNA with a 3' cytidine + CTP

\rightleftharpoons

a tRNA with a 3' CC end + diphosphate

(1c) a tRNA with a 3' CC end + ATP

\rightleftharpoons

a tRNA with a 3' CCA end + diphosphate

The acylation of all tRNAs with an amino acid occurs at the terminal ribose of a 3' CCA sequence.

References

Schofield P, Williams KR (August 1977). "Purification and some properties of Escherichia coli tRNA nucleotidyltransferase". The Journal of Biological Chemistry. 252 (15): 5584–8. PMID 328503.
Shi PY, Maizels N, Weiner AM (June 1998). "CCA addition by tRNA nucleotidyltransferase: polymerization without translocation?". The EMBO Journal. 17 (11): 3197–206. doi:10.1093/emboj/17.11.3197. PMC 1170658. PMID 9606201.
Augustin MA, Reichert AS, Betat H, Huber R, Mörl M, Steegborn C (May 2003). "Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization". Journal of Molecular Biology. 328 (5): 985–94. doi:10.1016/S0022-2836(03)00381-4. PMID 12729736.
Yakunin AF, Proudfoot M, Kuznetsova E, Savchenko A, Brown G, Arrowsmith CH, Edwards AM (August 2004). "The HD domain of the Escherichia coli tRNA nucleotidyltransferase has 2',3'-cyclic phosphodiesterase, 2'-nucleotidase, and phosphatase activities". The Journal of Biological Chemistry. 279 (35): 36819–27. doi:10.1074/jbc.M405120200. PMID 15210699.
Hou YM (April 2010). "CCA addition to tRNA: implications for tRNA quality control". IUBMB Life. 62 (4): 251–60. doi:10.1002/iub.301. PMC 2848691. PMID 20101632.

External links

CCA+tRNA+nucleotidyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

Transferases: phosphorus-containing groups (EC 2.7)

2.7.1-2.7.4:
phosphotransferase/kinase
(PO₄)

2.7.1: OH acceptor	Hexo- Gluco- Fructo- Hepatic Galacto- Phosphofructo- 1 Liver Muscle Platelet 2 Riboflavin Shikimate Thymidine ADP-thymidine NAD Glycerol Pantothenate Mevalonate Pyruvate Deoxycytidine PFP Diacylglycerol Phosphoinositide 3 Class I PI 3 Class II PI 3 Sphingosine Glucose-1,6-bisphosphate synthase
2.7.2: COOH acceptor	Phosphoglycerate Aspartate kinase
2.7.3: N acceptor	Creatine
2.7.4: PO₄ acceptor	Phosphomevalonate Adenylate Nucleoside-diphosphate Uridylate Guanylate Thiamine-diphosphate

2.7.6: diphosphotransferase
(P₂O₇)

2.7.7: nucleotidyltransferase
(PO₄-nucleoside)

Polymerase

DNA polymerase

DNA-directed DNA polymerase

I/A

Taq

II/B

Pfu

III/C

IV/X

TDT

V/Y

RNA-directed DNA polymerase

Reverse transcriptase

Telomerase

RNA polymerase

Template-directed

RNA-dependent RNA polymerase

Polyadenylation

PAP

PNPase

Phosphorolytic
3' to 5' exoribonuclease

Nucleotidyltransferase

Guanylyltransferase

mRNA capping enzyme

Other

2.7.8: miscellaneous

Phosphatidyltransferases	CDP-diacylglycerol—glycerol-3-phosphate 3-phosphatidyltransferase CDP-diacylglycerol—serine O-phosphatidyltransferase CDP-diacylglycerol—inositol 3-phosphatidyltransferase CDP-diacylglycerol—choline O-phosphatidyltransferase
Glycosyl-1-phosphotransferase	N-acetylglucosamine-1-phosphate transferase

2.7.10-2.7.13: protein kinase
(PO₄; protein acceptor)

2.7.10: protein-tyrosine	see tyrosine kinases
2.7.11: protein-serine/threonine	see serine/threonine-specific protein kinases
2.7.12: protein-dual-specificity	see serine/threonine-specific protein kinases
2.7.13: protein-histidine	Protein-histidine pros-kinase Protein-histidine tele-kinase Histidine kinase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Portal:

Biology

Category:

EC 2.7.7

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